Express Transfer Buffer
Quick Overview for Express Transfer Buffer (ABIN5706384)
应用范围
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Supplier Product No.
- mb-024-1000
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Supplier
- Rockland
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原理
- Express Transfer Buffer
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纯化方法
- This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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过滤
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: Express Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 X
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储存条件
- RT,4 °C
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储存方法
- Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Expiration date is three (3) months from date of receipt.
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有效期
- 3 months
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背景
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Synonyms: Carbonate Buffer, Western Blot Buffer, non-tris-glycine buffer
Background: Western Blotting is the method used for immunodetection for the detection of proteins. Blotting is a method for transferring biological molecules (e.g., proteins, nucleic acid fragments) from a gel matrix to a membrane support for the subsequent detection
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